chronic morphine consumption increase allograft rejection rate in rat through inflammatory reactions

نویسندگان

علی رفعتی

ali rafati سمیه حمزه ای تاج

sommaye hamzehie taj نگار آذرپیرا

negar azarpira اسدالله ظریفکار

assadollah zarifkar علی نورافشان

چکیده

background: although opioids suppressive effects on immune system function have been reported, this study demonstrates inflammatory reactions, such as production of pro-inflammatory cytokines and suppression of anti-inflammatory cytokines, are the main causes at organ’s allotransplantation rejection in chronic morphine-treated recipients. methods: 28 rats were categorized in 4groups through intra-peritoneal administrations: control, sham, morphine treated animals (20 mg/kg injected of morphine daily until biopsy day), morphine and naloxane treated animals (20 mg/kg morphine and 2 mg/kg naloxane daily injected until biopsy day), which their donors were normal rats. the grafts were done at the 14th day of the experiment. plasma interleukins levels (il-6 and il-10) in three sampling times were measured by elisa. with almost 80% of macroscopic rejection signs in rats of one group, full thickness skin biopsy has been taken and histological parameters like perivascular infiltrates, epidermal changes, and stromal changes were detected. the statistical significance differences between the control and experimental groups were analyzed using the kruskal-wallis, followed by anova post hoc test. results: accelerated skin allograft rejection by chronic morphine consumption can be resulted of increased il-6 concentration and decreased il-10. the enhancing effects of morphine on the graft inflammation were partially antagonized by naloxane. it can illustrate the complexity of opiates and immune system connections and should be considered during organ transplantation of opiate addicts. conclusion: expansion of skin cells in recipient with chronic morphine administration history may be resulted in failure.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Chronic Morphine Consumption Increase Allograft Rejection Rate in Rat through Inflammatory Reactions

Background: Although opioids suppressive effects on immune system function have been reported, this study demonstrates inflammatory reactions, such as production of pro-inflammatory cytokines and suppression of anti-inflammatory cytokines, are the main causes at organ’s allotransplantation rejection in chronic morphine-treated recipients. Methods: 28 rats were categorized in 4groups through int...

متن کامل

Chronic morphine consumption increase allograft rejection rate in rat through inflammatory reactions.

BACKGROUND Although opioids suppressive effects on immune system function have been reported, this study demonstrates inflammatory reactions, such as production of pro-inflammatory cytokines and suppression of anti-inflammatory cytokines, are the main causes at organ's allotransplantation rejection in chronic morphine-treated recipients. METHODS 28 rats were categorized in 4 groups through in...

متن کامل

A model of chronic lung allograft rejection in the rat.

Bronchiolitis obliterans, the pathological hallmark of chronic pulmonary rejection, severely impacts long-term survival following lung transplantation. However, experimental reproduction of this pathophysiological phenomenon has not been achieved with contemporary in vivo models. Here, a model of chronic rejection is described, with sensitised recipients receiving unilateral orthotopic rat lung...

متن کامل

Hyperlipidemia accelerates allograft arteriosclerosis (chronic rejection) in the rat.

The relevance of hyperlipidemia in allograft arteriosclerosis (chronic rejection) is controversial. Isolated hypercholesterolemia induced with cholesterol-cholic acid-diet (CC-diet) or hypertriglyceridemia induced with glycerol-diet (G-diet) had no or only a protective effect on aortic allograft arteriosclerosis in the rat. Combined hyperlipidemia with both diets (CC+G-diet) enhanced allograft ...

متن کامل

Donor simvastatin treatment abolishes rat cardiac allograft ischemia/reperfusion injury and chronic rejection through microvascular protection.

BACKGROUND Ischemia/reperfusion injury may have deleterious short- and long-term consequences for cardiac allografts. The underlying mechanisms involve microvascular dysfunction that may culminate in primary graft failure or untreatable chronic rejection. METHODS AND RESULTS Here, we report that rat cardiac allograft ischemia/reperfusion injury resulted in profound microvascular dysfunction t...

متن کامل

Cloning and characterization of allograft inflammatory factor-1: a novel macrophage factor identified in rat cardiac allografts with chronic rejection.

The development of arteriosclerotic lesions in the Lewis to F344 rat model of chronic cardiac rejection is characterized by macrophage adhesion to the vessel lumen and macrophage infiltration in the neointima prior to smooth muscle cell accumulation. We report the cloning and characterization of allograft inflammatory factor-1 (AIF-1), a novel cDNA that is expressed early and persistently in ch...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian biomedical journal

جلد ۱۵، شماره ۳، صفحات ۸۵-۹۱

کلمات کلیدی
[ ' m o r p h i n e ' , ' s k i n a l l o g r a f t ' , ' i n f l a m m a t i o n ' , ' i l ' , 6 , ' i l ' , 1 0 ]

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023